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In this review, we will take a historical view and highlight some of the milestones that had an important impact on the development of gene therapy. The precision and programmability of Cas9 is derived from its complexation with a guide-RNA gRNA that is complementary to a desired genomic sequence.

Significant advancements have been made in understanding the genetic pathogenesis of ocular diseases, and gene replacement and gene silencing have been implicated as potentially efficacious therapies. Preventing tumor recurrence requires systemic therapy ; however, current modalities are limited by toxicity or lack of efficacy.

We used a plasmid p VIVO 2 designed for in vivo gene delivery that produces minimal immune responses, has improved nuclear entry into post mitotic cells and also expresses green fluorescent protein GFP. The in vivo expression of apoptin and the induction of apoptosis were verified by reverse-transcription polymerase chain reaction analysis, TUNEL assay, and DAPI staining.

An inoculation of the irradiated IL induced chondrogenic cells significantly suppressed tumor by inducing tumor-specific cytotoxic T lymphocytes, enhancing natural killer tumoricidal activity and inhibiting tumor neoangiogenesis in the mice.

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